The mobile physical examination portable box is an integrated portable physical examination device provided to grassroots doctors. The whole device can collect and test electrocardiogram, blood pressure, pulse, body temperature, blood oxygen saturation, and urine routine, and supports the expansion of other equipment functions such as blood lipids, lung function, hemoglobin, height and weight. The follow-up system configured in the mobile physical examination portable box can meet the actual needs of grassroots doctors for physical examinations in rural households, online identification and file creation, and management of chronic disease populations.